LIPE antibody

Catalog No. ABIN317711

The Rabbit Polyclonal anti-LIPE antibody is suitable to detect LIPE in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for LIPE antibody (ABIN317711)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-LIPE Antibody

Specificity: This antibody detects endogenous levels of HSL protein.(region surrounding Ala546)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: LIPE

Background: HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.Synonyms: EC=3.1.1.79, HSL, Hormone-sensitive lipase, LIPE

Molecular Weight: approx. 115.0 kDa

Gene ID: 3991

NCBI Accession: NP_005348

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism