SCNN1D antibody

Catalog No. ABIN317816

The Rabbit Polyclonal anti-SCNN1D antibody is suitable to detect SCNN1D in samples from Human. It has been validated for WB and EIA.

Quick Overview for SCNN1D antibody (ABIN317816)

Target: SCNN1D (Sodium Channel, Nonvoltage-Gated 1, delta (SCNN1D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCNN1D antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SCNN1D Antibody

Specificity: This antibody detects endogenous levels of ENaCδ protein. (region surrounding Pro446)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SCNN1D

Target: SCNN1D (Sodium Channel, Nonvoltage-Gated 1, delta (SCNN1D))

Alternative Name: ENAC delta / SCNN1D

Background: The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that is located on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands and other organs. ENaC is formed by heteromultimerization of four homologous subunits, α, β, γ and δ. The most frequently formed heterotetramer consists of 2α, 1β, and 1γ subunit, but the α subunit can be replaced by a δ subunit. The αENaC gene maps to human chromosome 12p13, and expresses a glycosylated protein. Both the β and γENaC genes map to human chromosome 16p12, and the γENaC transcript is detected as a glycosylated protein. The carboxy-terminus of all ENaC subunits contains PY motifs, which interact with the ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-function mutations involving the PY motif cause Liddle's syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption. Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension.Synonyms: Amiloride-sensitive sodium channel subunit delta, DNACH, Delta-ENaC, Delta-NaCH, ENaCD, Epithelial Na(+) channel subunit delta, Nonvoltage-gated sodium channel 1 subunit delta, SCNED

Molecular Weight: approx. 80 kDa

Gene ID: 6339

NCBI Accession: NP_001123885

UniProt: P51172