Sodium Channel, Nonvoltage-Gated 1, delta (SCNN1D) antibody

Antigen: Sodium Channel, Nonvoltage-Gated 1, delta (SCNN1D)

Synonyms: ENaCd, SCNED, dNaCh, ENaCdelta, MGC149710, MGC149711, Scnn1d, SCNN1D

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN317816

Additional Information

Alternative name: ENAC delta / SCNN1D

Cross-Reactivity: Human

Description: The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that islocated on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distalcolon, lung, ducts of exocrine glands and other organs. ENaC is formed byheteromultimerization of four homologous subunits, α, β, γ and δ. The most frequentlyformed heterotetramer consists of 2α, 1β, and 1γ subunit, but the α subunit can bereplaced by a δ subunit. The αENaC gene maps to human chromosome 12p13, andexpresses a glycosylated protein. Both the β and γENaC genes map to human chromosome16p12, and the γENaC transcript is detected as a glycosylated protein. Thecarboxy-terminus of all ENaC subunits contains PY motifs, which interact with the ubiquitinprotein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-functionmutations involving the PY motif cause Liddle's syndrome, an autosomal dominant form ofhypertension, resulting from excessive renal sodium absorption. Conversely, ENaCloss-of-function mutations result in pseudohypoaldosteronism type I, a disordercharacterized by salt wasting and hypotension.

Specificity: ENaCδ antibody detects endogenous levels of ENaCδ protein. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1: 20000~1: 40000. WB: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Ion Channels

Restrictions: For Research Use only