MT-ND1 antibody

Catalog No. ABIN317922

The Rabbit Polyclonal anti-MT-ND1 antibody (ABIN317922) specifically detects MT-ND1 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for MT-ND1 antibody (ABIN317922)

Target: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MT-ND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MT-ND1 Antibody

Specificity: This antibody detects endogenous levels of ND1 protein.(region surrounding Val208)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MT-ND1

Target: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Alternative Name: ND1

Background: NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7),while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 1 (ND1) binds rotenone and rotenone analogs and might be involved in electron transfer to ubiquinone. Mutations in the ND1 gene may be implicated in several disorders, including Leber hereditary optic neuropathy, Alzheimer's disease and Parkinson disease.Synonyms: MT-ND1, MTND1, NADH-ubiquinone oxidoreductase chain 1, NADH1

Molecular Weight: approx. 40.0 kDa

Gene ID: 4535

NCBI Accession: YP_003024026

UniProt: P03886