BIK antibody (N-Term)

Catalog No. ABIN318742

This Rabbit Polyclonal antibody specifically detects BIK in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for BIK antibody (N-Term) (ABIN318742)

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-BIK Antibody

Binding Specificity: N-Term

Specificity: This antibody detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk).

Purification: Purified

Immunogen: 15 amino acid peptide from near the amino terminus of human Bik.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 - 2g/mL, detects a band of approximately 18 kDa in Jurkat cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: BIK

Background: Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis. Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.Synonyms: Apoptosis inducer NBK, BIP1, BP4, Bcl-2-interacting killer, NBK

Gene ID: 638

NCBI Accession: NP_001188

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity