IL32 antibody (Middle Region)

Catalog No. ABIN318795

The Rabbit Polyclonal anti-IL32 antibody (ABIN318795) specifically detects IL32 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for IL32 antibody (Middle Region) (ABIN318795)

Target: IL32 (Interleukin 32 (IL32))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL32 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL32 Antibody

Binding Specificity: Middle Region

Specificity: This antibody recognises IL-32.

Purification: Antisera to human IL-32 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen: A 16 amino acid peptide located near the centre of human IL-32.

Isotype: IgG

Application Details

Application Notes: Western Blot: 5 - 10g/mL, detects a band/s of approximately 26 kDa in jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for IL32

Target: IL32 (Interleukin 32 (IL32))

Alternative Name: Interleukin-32 / IL32

Background: IL-32 is a 26 kDa cytokine, thought to play a role in innate and adaptive immune responses. IL-32 is produced by mitogen-activated lymphocytes, interferon-gamma activated epithelial cells and IL-12, IL-18, IL-32-activated NK cells. It is expressed at elevated levels after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappa-B and p38 MAPK cytokine signal pathways. TNF-alpha, IL-1beta, IL-6 and 2 C-X-C chemokine family members, all involved in autoimmune and inflammatory diseases, including rheumatoid arthritis are induced by IL-32.Synonyms: IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor

Gene ID: 9235

NCBI Accession: NP_001012649

UniProt: P24001