EIF2S1 antibody (pSer49)

Catalog No. ABIN319276

The Rabbit Polyclonal anti-EIF2S1 antibody (ABIN319276) specifically detects EIF2S1 in WB, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EIF2S1 antibody (pSer49) (ABIN319276)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF2S1 Antibody

Binding Specificity: pSer49

Specificity: Antibody detects endogenous levels of eIF2a only when phosphorylated at Serine 49.

Purification: Affinity Chromatography

Immunogen: Synthetic phosphopeptide derived from human eIF2a around the phosphorylation site of Serine 49 (L-L-SP-E-L).

Application Details

Application Notes: Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2A / EIF2S1

Background: The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.Synonyms: EIF2-A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2 alpha

Gene ID: 1965

NCBI Accession: NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C