HA-Tag antibody

Catalog No. ABIN319423

This Rabbit Polyclonal antibody specifically detects HA-Tag in WB. It exhibits reactivity toward and has been mentioned in 2+ publications.

Quick Overview for HA-Tag antibody (ABIN319423)

Target: HA-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HA-Tag Antibody

Sequence: YPYDVPDYA

Purification: Immunoaffinity Chromatography using epitope-specific peptide.

Immunogen: The antiserum was produced by immunizing rabbit with synthesized peptide containing the Influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).

Application Details

Application Notes: Western Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50 % Glycerol, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

References for anti-HA-Tag antibody (ABIN319423)

Qiu, Ding, Zou, Tan, Liu, Fu, Xu: "Divergent roles of amino acid residues inside and outside the BB loop affect human Toll-like receptor (TLR)2/2, TLR2/1 and TLR2/6 responsiveness." in: PLoS ONE, Vol. 8, Issue 4, pp. e61508, (2013) (PubMed).

Giridharan, Cai, Vitale, Naslavsky, Caplan: "Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis." in: Molecular biology of the cell, Vol. 24, Issue 11, pp. 1776-90, S1-15, (2013) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Epitope Tag (YPYDVPDYA)

Target Type: Tag

Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.