KIT antibody
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- Target See all KIT Antibodies
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This KIT antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody reacts with CD117 also known as Kit or c-kit receptor. Clone 2B8 recognises an epitope within the extracellular region of CD117.
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- IL-3 dependent mast cells. Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
- Clone
- 2B8
- Isotype
- IgG2b
- Top Product
- Discover our top product KIT Primary Antibody
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anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 321-520) antibody
KIT Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 343-527) antibodyKIT Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 350-440) antibodyKIT Reactivity: Human, Mouse, Rat FACS, ELISA, IF (p), IF (cc), IHC (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibodyKIT Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr703) antibodyKIT Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (Internal Region) antibodyVerified KIT Reactivity: Human WB, FACS, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 546-976) antibodyKIT Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 2E3 unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 25-295) antibodyKIT Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) antibody (FITC)KIT Reactivity: Human, Cow, Non-Human Primate FACS Host: Mouse Monoclonal 104D2 FITC
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 106-135), (N-Term) antibodyKIT Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB15297 unconjugated
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- Application Notes
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Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- Alternative Name
- CD117 / C-Kit (KIT Products)
- Background
- CD117 is the receptor of the steel factor (Stem Cell Factor). C-kit receptor is expressed on 5-10 % of total adult bone marrow cells including multipotent hematopoietic cells.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
- Gene ID
- 16590
- NCBI Accession
- NP_066922
- UniProt
- P05532
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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