Macrophage/Monocyte antibody

Catalog No. ABIN319627

This Rat Monoclonal antibody specifically detects Macrophage/Monocyte in FACS and IHC (fro). It exhibits reactivity toward Mouse. It has been mentioned in 8+ publications

Quick Overview for Macrophage/Monocyte antibody (ABIN319627)

Target: Macrophage/Monocyte

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MOMA-2

Product Details

Specificity: MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with the anti F4/80 marker BM8 (product BM4007) it allows a precise characterisation of tissue fixed macrophages in various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface. MOMA-2 detects a (glyco-)protein of 140 kDa MW which is located within the cytoplasm and on the cell surface. Antigen Distribution Isolated Cells: In the cytospin preparation of thiogycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells. Tissue Sections: MOMA-2 detects typical tissue macrophages as does the anti F4/80 specific clone BM8. However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where the anti F4/80 clone BM8 is negative.Antigen Distribution

Cross-Reactivity (Details): Species reactivity (tested):Mouse (Monocytes and Macrophages).

Purification: Affinity Chromatography

Immunogen: Mouse lymph node stroma. The epitope has not been further characterized.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (membrane permeabilisation is recommended). Immunohistochemistry on fresh frozen sections: fixed with dry acetone at 4 °C, use at 0.5-1 μg/mL (1:400-1:800). Suggested positive control: mouse spleen. Does not work on formalin-fixed, paraffin-embedded tissue sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: The use of commercially available secondary antibodies that have been preadsorbed against mouse serum proteins may yield a weak staining.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).

Concentration: 0.4 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!

References

Asquith, Stauffer, Davin, Mitchell, Lin, Rosenbaum: "Perturbed Mucosal Immunity and Dysbiosis Accompany Clinical Disease in a Rat Model of Spondyloarthritis." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 68, Issue 9, pp. 2151-62, (2017) (PubMed).

Nishibayashi, Inoue, Harada, Watanabe, Makioka, Ushida: "RNA of Enterococcus faecalis Strain EC-12 Is a Major Component Inducing Interleukin-12 Production from Human Monocytic Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129806, (2016) (PubMed).

Movita, Kreefft, Biesta, van Oudenaren, Leenen, Janssen, Boonstra: "Kupffer cells express a unique combination of phenotypic and functional characteristics compared with splenic and peritoneal macrophages." in: Journal of leukocyte biology, Vol. 92, Issue 4, pp. 723-33, (2012) (PubMed).

Heigele, Schindler, Gnanadurai, Leonard, Collins, Kirchhoff: "Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins." in: Journal of virology, Vol. 86, Issue 1, pp. 36-48, (2012) (PubMed).

Schreiber, Taschler, Wolinski, Seper, Tamegger, Graf, Kohlwein, Haemmerle, Zimmermann, Zechner, Lass: "Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver." in: Journal of lipid research, Vol. 50, Issue 12, pp. 2514-23, (2009) (PubMed).

Luchtefeld, Schunkert, Stoll, Selle, Lorier, Grote, Sagebiel, Jagavelu, Tietge, Assmus, Streetz, Hengstenberg, Fischer, Mayer, Maresso, El Mokhtari, Schreiber, Müller, Bavendiek, Grothusen, Drexler et al.: "Signal transducer of inflammation gp130 modulates atherosclerosis in mice and man. ..." in: The Journal of experimental medicine, Vol. 204, Issue 8, pp. 1935-44, (2007) (PubMed).

Saaristo, Tammela, Farkkil?, Kärkkäinen, Suominen, Yla-Herttuala, Alitalo: "Vascular endothelial growth factor-C accelerates diabetic wound healing." in: The American journal of pathology, Vol. 169, Issue 3, pp. 1080-7, (2006) (PubMed).

Leithäuser, Bäuerle, Huynh, Möller: "Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma." in: Blood, Vol. 98, Issue 9, pp. 2762-70, (2001) (PubMed).

Target Details

Target: Macrophage/Monocyte

Alternative Name: Macrophages / Monocytes

Background: Monocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.Synonyms: Macrophage marker, Monocyte marker