FCGR2A antibody
Quick Overview for FCGR2A antibody (ABIN319715)
Target
See all FCGR2A AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- This antibody recognises the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose.
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Purification
- Affinity Chromatography on Protein A.
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Immunogen
- K562 cell line. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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Isotype
- IgG1
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anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibody
FCGR2A Reactivity: Human WB, IF, IHC, IP, IHC (p) Host: Mouse Monoclonal 9C6 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A Reactivity: Human, Monkey WB, IHC, IP, IHC (p) Host: Mouse Monoclonal 20D1 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 241-317) antibodyFCGR2A Reactivity: Human WB, ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (C-Term) antibodyFCGR2A Reactivity: Human WB, ELISA, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A Reactivity: Human WB, IF, IHC, IP, IHC (p), ICC Host: Mouse Monoclonal 6H7 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-316) antibodyFCGR2A Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 1-316) antibodyFCGR2A Reactivity: Human WB Host: Mouse Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 46-150) antibodyFCGR2A Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3E8 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 256-285), (C-Term) antibodyFCGR2A Reactivity: Human WB Host: Rabbit Polyclonal RB33694 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 255-282), (C-Term) antibodyFCGR2A Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB23549 unconjugated
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Application Notes
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Immunoprecipitation: 20 μg/mL. Flow Cytometry (20 μg/mL): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Recommended Positive Control: Lymph node.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
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Alternative Name
- CD32 / FcRII-A
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Background
- CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
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Gene ID
- 2212
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NCBI Accession
- NP_001129691
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UniProt
- P12318
Target
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