Histiocytes antibody

Catalog No. ABIN319872

The Mouse Monoclonal anti-Histiocytes antibody (Clone 3A5) (ABIN319872) specifically detects Histiocytes in IHC (fro) and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Histiocytes antibody (ABIN319872)

Target: Histiocytes

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3A5

Product Details

Specificity: This antibody recognises an antigen expressed by human macrophages, monocytes and histiocytes, but does not stain interdigitating dendritic cells, Langerhans cells, microglial cells, or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen. In immunohistochemical studies the antibody shows partial cross-blocking with some anti-CD68 monoclonal antibodies, suggesting that the epitope may be associated with this antigen.

Purification: Tissue Culture Supernatant serum free

Immunogen: Human spleen cell homogenate, depleted of lymphocytes and erythrocytes

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Sections: 1/10-1/100, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Contol: Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains 0.09 % Sodium Azide as preservative and 0.7 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Histiocytes