Integrin Alpha2b antibody

Catalog No. ABIN320116

Product Details anti-Integrin Alpha2b Antibody

Target: Integrin Alpha2b (CD41)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin Alpha2b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Specificity: This antibody recognizes the integrin alpha IIb subunit CD41. Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo (Nieswandt et al., 1999). SM2035A is recommended for use in functional studies.

Purification: Affinity Chromatography on Protein G.

Clone: MWReg30

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1/50-1/100): Use 10 μL to label 10^6 cells in 100 μL. Immunohistology on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References for anti-Integrin Alpha2b antibody (ABIN320116)

Koski, Bramante, Kipar, Oksanen, Juhila, Vassilev, Joensuu, Kanerva, Hemminki: "Biodistribution Analysis of Oncolytic Adenoviruses in Patient Autopsy Samples Reveals Vascular Transduction of Noninjected Tumors and Tissues." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 10, pp. 1641-52, (2016) (PubMed).

Pennings, Siljee, Imholz, Kuc, de Vries, Schielen, Rodenburg: "Comparison of different blood collection, sample matrix, and immunoassay methods in a prenatal screening setting." in: Disease markers, Vol. 2014, pp. 509821, (2015) (PubMed).

Teeling, Carare, Glennie, Perry: "Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction." in: Acta neuropathologica, Vol. 124, Issue 4, pp. 479-90, (2012) (PubMed).

Haglund, Tillgren, Addis, Wenglén, Recklies, Heinegård: "Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment." in: The Journal of biological chemistry, Vol. 286, Issue 5, pp. 3925-34, (2011) (PubMed).

Sullivan, Wang, Tawfik, Luyendyk: "Protective and damaging effects of platelets in acute cholestatic liver injury revealed by depletion and inhibition strategies." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 1, pp. 286-94, (2010) (PubMed).

Leung, Greene, Ni, Raymond, Lehnert, Langley, Krissansen: "Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts." in: Immunology and cell biology, Vol. 74, Issue 6, pp. 490-6, (1997) (PubMed).

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: CD41 / ITGA2B (CD41 Products)

Background: CD41, a ~125 kDa single pass type 1 transmembrane glycoprotein, is expressed on platelets, megakaryocytes, mast cells and hematopoietic progenitors and forms a heterodimer with CD61. CD41/CD61 is important for platelet adhesion and aggregation.Synonyms: GP2B, GPalpha IIb, ITGAB, Integrin alpha-IIb, Platelet membrane glycoprotein IIb

Gene ID: 16399

NCBI Accession: NP_034705

UniProt: Q9QUM0

Pathways: Integrin Complex