Integrin Alpha2b antibody (CD41)

Details for Product anti-CD41 Antibody No. ABIN94111
Antigen
Reactivity
Human, Non-Human Primate
369
95
28
16
11
9
5
4
2
2
2
1
1
1
1
1
Host
Mouse
298
76
60
Clonality (Clone)
Monoclonal ()
Conjugate
This Integrin Alpha2b antibody is un-conjugated
59
42
41
15
10
6
5
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
306
93
75
71
56
49
33
20
20
13
7
6
5
3
3
1
1
1
Options
Clone HIP8
Isotype IgG1
Specificity The antibody HIP8 reacts with alpha (a) subunit of CD41 (heavy chain, 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
The antibody HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies of platelets and megakaryocytes.
HLDA IV, WS Code P 38
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Background CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Pathways Integrin Complex
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Wu: "[Production and application of anti-human platelet GP IIb/IIIa monoclonal antibodies]." in: Zhonghua yi xue za zhi, Vol. 69, Issue 8, pp. 427-30, 30, 1990 (PubMed).