TGFBR3 antibody (Extracellular Domain)

Catalog No. ABIN326173

This Goat Polyclonal antibody specifically detects TGFBR3 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for TGFBR3 antibody (Extracellular Domain) (ABIN326173)

Target: TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFBR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TGFBR3 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human TGFb RIII. Based on direct ELISA and Western Blot results, this antibody shows less than 1 % cross-reactivity with rhTGFb RII.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rhTGFb sRIII extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Tested in flow cytometry using HeLa cells. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 0.16 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1.25 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Storage: -20 °C

Storage Comment: Reconstitute with sterile PBS and store at -20°C. Aliquot to avoid freeze-thaw cycles.

Target Details for TGFBR3

Target: TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))

Alternative Name: Betaglycan / TGFBR3

Background: Name/Gene ID: TGFBR3

Synonyms: TGFBR3, Betaglycan, BGCAN, TGF-beta receptor type 3, TGF-beta receptor type III, TGFR-3, Betaglycan proteoglycan, Tgf-beta receptors type iii

Gene ID: 7049

UniProt: Q03167