IL9 Receptor antibody (Extracellular Domain)

Catalog No. ABIN327384

Product Details anti-IL9 Receptor Antibody

Target: IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL9 Receptor antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Functional Studies (Func), Neutralization (Neut)

Specificity: Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.

Purification: Protein G purified

Immunogen: Sf21-derived rhIL-9 R extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, Func, Neut

Usage: Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 μg/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for IL9 Receptor

Target: IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))

Alternative Name: IL9R / CD129 (IL9R Products)

Background: Name/Gene ID: IL9R
Family: Interleukin

Synonyms: IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen

Gene ID: 3581

UniProt: Q01113

Pathways: JAK-STAT Signaling, Growth Factor Binding