ICOSLG antibody

Catalog No. ABIN331017

The Mouse Monoclonal anti-ICOSLG antibody (ABIN331017) specifically detects ICOSLG in WB, ELISA, FACS and Neut. The antibody is reactive with Human samples.

Quick Overview for ICOSLG antibody (ABIN331017)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Product Details anti-ICOSLG Antibody

Specificity: Recognizes human ICOS Ligand. In direct ELISAs and Western Blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, or rmB7-H2.

Purification: Protein G purified

Immunogen: NS0-derived rhICOS Ligand.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Neutralization, Flow Cytometry, Western Blot and Direct ELISA. Neutralization: ~1-4 μg/mL Dependent on cell type, growth conditions, activity studied. ND50 in the presence of 20 ng/mL of anti-human CD3 and 3 μg/mL of recombinant human B7-H2/Fc, using 72 hours PHA-stimulated human T cells. Flow Cytometry: 25 μg/mL, add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in 200 μL. ELISA: 0.5-1 μg/mL, limit ~3 ng/well. Western Blot: 1-2 μg/mL, limit ~25 ng/lane.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: ICOSLG / ICOSL / ICOS Ligand

Background: Name/Gene ID: ICOSLG

Synonyms: ICOSLG, B7RP1, B7 homolog 2, B7 homologue 2, B7-H2, B7H2, B7RP-1, CD275 antigen, ICOS ligand, GL50, ICOS-L, ICOSL, KIAA0653, B7-like protein Gl50, B7-related protein 1, CD275, LICOS

Gene ID: 23308

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation