C4b-Binding Protein antibody

Antigen: C4b-Binding Protein

Clonality: Monoclonal (10-07)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN334259

Additional Information

Alternative name: C4b-binding Protein (C4BP)

Immunogen: Native human C4 binding protein.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 10-07

Description: Gene: C4b-binding Protein (C4BP)

Specificity: Recognizes complement component 4 binding protein (C4bp). There are three variants of C4bp, composed of alpha and beta chains. The predominant form is a 570 kD complex of 7 alpha chains and 1 beta chain. The other complexes formed are a 530 kD homoheptamer of alpha chains or a 500 kD complex of 6 alpha chains and 1 beta chain. C4bp regulates the activation of the classical complement pathway. It is a cofactor to the C3b/C4b inactivator, crucial to the proteolytic cleavage of C4b and important in cleavage of C3b. C4bp also regulates the C4bC2a complex (C3 convertase), accelerating its degradation by dissociating the complement fragment C2a. Both the C4bp alpha and beta chains interact with anticoagulant protein S and C4bp alpha binds protein S. C4bp is expressed in the blood serum.

Application Details

Application Notes: ELISA, IHC-P (5 µg/ml)

Concentration: 1 mg/ml

Purification: Protein A Column

Buffer: Borate buffered saline, pH 8.4, 0.02% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.

Restrictions: For Research Use only

Images

Human Liver (formalin-fixed, paraffin-embedded) stained with C4BPA antibody ABIN334259 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.