ATP-Binding Cassette, Sub-Family D (Ald), Member 1 (ABCD1) antibody

Antigen: ATP-Binding Cassette, Sub-Family D (Ald), Member 1 (ABCD1)

Synonyms: ALD, AMN, ALDP, ABC42, Ald, Aldgh, RGD1562128, ABCD1, MGC129031, abcd1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334339

Additional Information

Alternative name: ABCD1

Gene ID: 215

Immunogen: C-EDMQRKGYSEQD

Description: All names and symbols: ABCD1, ATP-binding cassette, sub-family D (ALD), member 1, ABC42, ALD, ALDP, AMN, adrenoleukodystrophy protein. Official Symbol: ABCD1. RefSeq number(s): NP_000024.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx. 50kDa band in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 82.9kDa according to NP_000024.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Metabolism, Organelles

Restrictions: For Research Use only

Images

HEK293 overexpressing human ABCD1 and probed with ABIN334339 at 1ug/ml (mock transfection in first lane).

Publications

Publications: Hoeftberger, Kunze, Weinhofer et al.: "Distribution and cellular localization of adrenoleukodystrophy protein in human tissues: implications for X-linked adrenoleukodystrophy." in: Neurobiology of disease, Vol. 28, Issue 2, pp. 165-74, 2007 (PubMed).