Acylglycerol Kinase (AGK) antibody

Antigen: Acylglycerol Kinase (AGK)

Synonyms: MULK, FLJ10842, Mulk, AI465370, 2610037M15Rik, 6720408I04Rik, mulk, fi38e09, zgc:55462, wu:fi38e09, MGC52920, RGD1562046, MGC157181, DKFZp459M1943

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334340

Additional Information

Alternative name: Acylglycerol kinase

Gene ID: 55750, 69923 (mouse)

Immunogen: CDPRKREQMLTSP

Description: All names and symbols: acylglycerol kinase, FLJ10842 , MULK, multi-substrate lipid kinase , multiple substrate lipid kinase, AGK. Official Symbol: AGK. RefSeq number(s): NP_060708.1

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334340 (1µg/ml) staining of Human Brain (Substantia nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Outeiro, Kontopoulos, Altmann et al.: "Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's disease." in: Science (New York, N.Y.), Vol. 317, Issue 5837, pp. 516-9, 2007 (PubMed).