Apolipoprotein C-I (APOC1) antibody

Antigen: Apolipoprotein C-I (APOC1)

Synonyms: ALPCI, LRRG04, APOC1, apoc1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN334349

Additional Information

Alternative name: APOC1

Gene ID: 341

Immunogen: Peptide with sequence C-ETFQKVKEKLKIDS, from the C Terminus of the protein sequence according to NP_001636.1

Description: All names and symbols: APOC1, apolipoprotein C-I. Official Symbol: APOC1. RefSeq number(s): NP_001636.1.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave bands at 30kDa and 37kDa band in Human Liver lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.3kDa according to NP_001636.1. The detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Liver shows spotty staining in the cytoplasm of hepatocytes. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Berbee, van der Hoogt, Sundararaman et al.: "Severe hypertriglyceridemia in human APOC1 transgenic mice is caused by apoC-I-induced inhibition of LPL." in: Journal of lipid research, Vol. 46, Issue 2, pp. 297-306, 2005 (PubMed).