Brain and Acute Leukemia, Cytoplasmic (BAALC) antibody

Antigen: Brain and Acute Leukemia, Cytoplasmic (BAALC)

Synonyms: FLJ12015, 2810457D07Rik, si:zc215i13.3, si:ch211-215i13.3, BAALC, MGC142796

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334357

Additional Information

Alternative name: BAALC

Gene ID: 79870

Immunogen: C-KQNGLQTTEAKRDAK

Description: All names and symbols: BAALC, brain and acute leukemia, cytoplasmic , HGNC:14333, FLJ12015. Official Symbol: BAALC. RefSeq number(s): NP_079088.1

Specificity: This antibody is expected to recognise isoform 1 (NP_079088.1) only. Human, Mouse, Rat, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 18kDa band observed in mouse brain lysates (calculated MW of 15.6kDa according to human NP_079088.1 and of 15.5kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1µg/ml. An additional band of unknown identity was also consistently observed at 48kDa in both Human and Mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334357 (0.03µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Satoskar, Tanner, Weinstein et al.: "Baalc, a marker of mesoderm and muscle." in: Gene expression patterns : GEP, Vol. 5, Issue 4, pp. 463-73, 2005 (PubMed).