BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) antibody

Antigen: BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1)

Synonyms: NIP1, SEC20, TRG-8

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334362

Additional Information

Alternative name: BNIP1

Gene ID: 662, 224630 (mouse), 140932 (rat)

Immunogen: Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1.

Description: All names and symbols: BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1. Official Symbol: BNIP1. RefSeq number(s): NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1

Specificity: All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1) Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

ABIN334362 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Nakajima, Hirose, Taniguchi et al.: "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." in: The EMBO journal, Vol. 23, Issue 16, pp. 3216-26, 2004 (PubMed).