BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) antibody
| Antigen |
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| Synonyms |
NIP1, SEC20, TRG-8 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN334362 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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BNIP1
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Gene ID
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662, 224630 (mouse), 140932 (rat)
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Immunogen
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Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1.
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Description
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All names and symbols: BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1. Official Symbol: BNIP1. RefSeq number(s): NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1
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Specificity
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All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1) Human, Mouse, Rat
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Signaling, Apoptosis/Necrosis
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Restrictions
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For Research Use only
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Nakajima, Hirose, Taniguchi et al.: "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." in: The EMBO journal, Vol. 23, Issue 16, pp. 3216-26, 2004 (PubMed).
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Alternatives for antigen "BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (5), Goat (3)
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Reactivities
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Human (40), Mouse (Murine) (23), Rat (Rattus) (23), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1)
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Applications
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Western Blotting (WB) (25), Immunofluorescence (IF) (16), ELISA (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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AA 1-199 (3), BH3 Domain (2), C-Term (2), N-Term (2), AA 1-229 (1), AA 150-170 (1), Internal Region (1), Isoform 1 (1), N-Term,AA 26-55 (1)
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Alternatives 
