Chromobox Homolog 8 (CBX8) antibody

Antigen: Chromobox Homolog 8 (CBX8)

Synonyms: PC3, RC1, HPC3, cbx8, MGC147589

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334369

Additional Information

Alternative name: CBX8

Gene ID: 57332, 30951 (mouse)

Immunogen: C-RERGTSRVDDKPSS

Description: All names and symbols: CBX8, chromobox homolog 8 (Pc class homolog, Drosophila) , HPC3, PC3, RC1, chromobox homolog 8, polycomb 3. Official Symbol: CBX8 . RefSeq number(s): NP_065700.1

Specificity: Human, Mouse, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 40kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_065700.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334369 (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Dietrich, Bracken, Trinh et al.: "Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus." in: The EMBO journal, Vol. 26, Issue 6, pp. 1637-48, 2007 (PubMed).