Estrogen-Related Receptor gamma (ESRRG) antibody

Antigen: Estrogen-Related Receptor gamma (ESRRG)

Synonyms: ERR3, NR3B3, ERRgamma, FLJ16023, KIAA0832, DKFZp781L1617, Errg, mKIAA0832, errg, errgamma, errb/g, esrrgl, errbeta/gamma, ESRRG, USH2A, err3, nr3b3, LOC100125509, DKFZp459J0417, LOC100219115, LOC100230542, errg2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334393

Additional Information

Alternative name: ESRRG

Gene ID: 2104, 26381 (mouse)

Immunogen: CRMSNKDRHIDS

Description: All names and symbols: ESRRG, DKFZp781L1617, Estrogen-related receptor gamma, ERR3, FLJ16023, KIAA0832, NR3B3. Official Symbol: ESRRG. RefSeq number(s): NP_001429.2, NP_996317.1, NP_996318.1

Specificity: This antibody is expected to recognise the reported isoform NP_001429.2. Human, Mouse, Dog

Synonyms: ERR3, NR3B3, ERRgamma, FLJ16023, KIAA0832, DKFZp781L1617, Errg, mKIAA0832, errg, errgamma, errb/g, esrrgl, errbeta/gamma, ESRRG, USH2A, err3, nr3b3, LOC100125509, DKFZp459J0417, LOC100219115, LOC100230542, errg2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 50kDa band observed in Human Brain (Cerebellum) and Breast lysates (calculated MW of 51.3kDa according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Hormones, Receptors

Restrictions: For Research Use only

Images

ABIN334393 (0.2µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ESRRG (RC218233) and probed with ABIN334393 (mock transfection in first lane), tested by Origene.

Publications

Publications: Dufour, Wilson, Huss et al.: "Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma." in: Cell metabolism, Vol. 5, Issue 5, pp. 345-56, 2007 (PubMed).