Extended Synaptotagmin-Like Protein 1 (ESYT1) antibody

Antigen: Extended Synaptotagmin-Like Protein 1 (ESYT1)

Synonyms: Mbc2, vp115, Fam62a, MGC124625, FAM62A, MGC139353, fam62a, si:ch211-239j9.4, DKFZp459I1628, MBC2, KIAA0747

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334395

Additional Information

Alternative name: FAM62A

Gene ID: 23344, 23943 (mouse), 29579 (rat)

Immunogen: C-QKKRTLSPEFNER

Description: All names and symbols: FAM62A, family with sequence similarity 62 (C2 domain containing), member A, KIAA0747, MBC2, membrane bound C2 domain containing protein, vp115. Official Symbol: FAM62A. RefSeq number(s): NP_056107.1

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 150kDa band observed in Human Adipose lysates (calculated MW of 123kDa according to NP_056107.1). In transfected HEK293 transiently expressing FAM62A a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334395 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing FAM62A (RC201380) and probed with ABIN334395 (mock transfection in first lane), tested by Origene.

Publications

Publications: Morris, Ross, Neveu et al.: "Cloning and preliminary characterization of a 121 kDa protein with multiple predicted C2 domains." in: Biochimica et biophysica acta, Vol. 1431, Issue 2, pp. 525-30, 1999 (PubMed).