Forkhead Box P2 (FOXP2) (internal) antibody

Antigen: Forkhead Box P2 (FOXP2)

Synonyms: SPCH1, CAGH44, TNRC10, DKFZp686H1726, CAG-16, D0Kist7, AI449000, 2810043D05Rik, FOXP2, RGD1559697, spch1, cagh44, tnrc10, xlFoxP2, foxP2, foxP, Foxp2, foxp1c

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334400

Additional Information

Alternative name: FOXP2 (internal)

Gene ID: 93986, 114142 (mouse)

Immunogen: C-DEVEYQKRRSQKIT

Description: All names and symbols: FOXP2, forkhead box P2 , CAGH44, DKFZp686H1726, SPCH1, TNRC10 , CAG repeat protein 44, forkhead/winged-helix transcription factor, speech and language disorder 1, trinucleotide repeat containing 10. Official Symbol: FOXP2. RefSeq number(s): NP_055306.1, NP_683696.1, NP_683697.1, NP_683698.1

Specificity: This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.1) and isoform III (NP_683697.1 and NP_683698.1). Human, Mouse, Rat, Dog 715 aa, 740 aa, 623 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334400 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lai, Fisher, Hurst et al.: "A forkhead-domain gene is mutated in a severe speech and language disorder." in: Nature, Vol. 413, Issue 6855, pp. 519-23, 2001 (PubMed).