G Protein-Coupled Receptor 3 (GPR3) antibody

Antigen: G Protein-Coupled Receptor 3 (GPR3)

Synonyms: ACCA, Gpcr3, GPR3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334411

Additional Information

Alternative name: GPR3

Gene ID: 2827, 14748 (mouse), 266769 (rat)

Immunogen: C-LQRHLLPASH

Description: All names and symbols: GPR3, G protein-coupled receptor 3 , ACCA, OTTHUMP00000043209, adenylate cyclase constitutive activator. Official Symbol: GPR3. RefSeq number(s): NP_005272.1

Specificity: This antibody is not expected to cross-react with GPR6 and GPR12. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human Brain (Hippocampus and Frontal Cortex) lysates (calculated MW of 35.0kDa according to NP_005272.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334411 (0.3µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Tanaka, Ishii, Kasai et al.: "Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth." in: The Journal of biological chemistry, Vol. 282, Issue 14, pp. 10506-15, 2007 (PubMed).