HIV-1 Tat Specific Factor 1 (HTATSF1) antibody

Antigen: HIV-1 Tat Specific Factor 1 (HTATSF1)

Synonyms: TAT-SF1, dJ196E23.2, 1600023H17Rik, 2600017A12Rik, 2700077B20Rik, RGD1563377, MGC66252, zgc:66252, MGC98898, HTATSF1, DKFZp468B086

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334420

Additional Information

Alternative name: HTATSF1

Gene ID: 27336, 317612 (rat)

Immunogen: C-QELYGDGKDGDTQTD

Description: All names and symbols: HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription. Official Symbol: HTATSF1. RefSeq number(s): NP_055315.2

Specificity: Human, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25,274(5287):605-10., PMID: 8849451). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334420 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zhou, Deng, Lacoste et al.: "Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription." in: Journal of virology, Vol. 78, Issue 24, pp. 13522-33, 2004 (PubMed).