Lysophospholipase-Like Protein 1 (LYPLAL1) antibody

Antigen: Lysophospholipase-Like Protein 1 (LYPLAL1)

Synonyms: FLJ99730, KIAA1238, Q96AVO, BC027340, MGC28394, MGC159517, DKFZp469J1534

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334430

Additional Information

Alternative name: LYPLAL1

Gene ID: 127018

Immunogen: DRFKITNDCPEHLE

Description: All names and symbols: LYPLAL1, lysophospholipase-like 1, KIAA1238. Official Symbol: LYPLAL1. RefSeq number(s): NP_620149.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28kDa band observed in Human Adipose and Heart lysates (calculated MW of 26.3kDa according to NP_620149.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Images

ABIN334430 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Steinberg, Kemp, Watt: "Adipocyte triglyceride lipase expression in human obesity." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 4, pp. E958-64, 2007 (PubMed).