Methyl-CpG Binding Domain Protein 2 (MBD2) (isoform 1) antibody

Antigen: Methyl-CpG Binding Domain Protein 2 (MBD2)

Synonyms: DMTase, NY-CO-41, DKFZp586O0821, MBD2a, MGC111862, wu:fa97f06, zgc:111862, MBD2, MGC188552

Epitope: isoform 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334432

Additional Information

Alternative name: MBD2 (isoform 1)

Gene ID: 8932, 17191 (mouse)

Immunogen: C-RNDPLNQNKGKPDLN

Description: All names and symbols: MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41. Official Symbol: MBD2. RefSeq number(s): NP_003918.1

Specificity: This antibody is expected to resognize isoform 1 (NP_003918.1) only. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct,26(19):7224-35., PMID: 16980624). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334432 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Auriol, Billard, Magdinier et al.: "Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus." in: Nucleic acids research, Vol. 33, Issue 13, pp. 4243-54, 2005 (PubMed).