Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) antibody

Antigen: Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9)

Synonyms: CAS2, CASL, HEF1, CAS-L, CASS2, dJ49G10.2, dJ761I2.1, NEDD9

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334443

Additional Information

Alternative name: NEDD9

Gene ID: 4739, 18003 (mouse), 291044 (rat)

Immunogen: NKPQNKCDDLDR

Description: All names and symbols: NEDD9, neural precursor cell expressed, developmentally down-regulated 9, CAS-L, CASL, HEF1, dJ49G10.2, dJ761I2.1, Crk-associated substrate related, cas-like docking dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)), dJ761I2.1 (enhancer of filamentation (HEF1)), enhancer of filamentation 1. Official Symbol: NEDD9. RefSeq number(s): NP_006394.1, NP_892011.2

Specificity: This antibody is expected to recognise isoform 1 (NP_006394.1) only. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 100kDa band observed in Human Kidney, Lung and Placenta lysates (calculated MW of 92.9kDa according to NP_006394.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cell Cycle, Signaling

Restrictions: For Research Use only

Images

ABIN334443 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kim, Gans, Nogueira et al.: "Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene." in: Cell, Vol. 125, Issue 7, pp. 1269-81, 2006 (PubMed).