Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (AA 247-257) antibody

Antigen: Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1)

Synonyms: P2Y1, P2y, P2y1, P2RY1

Epitope: AA 247-257

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334454

Additional Information

Alternative name: P2RY1 (aa247-257)

Gene ID: 5028

Immunogen: C-KDLDNSPLRRK

Description: All names and symbols: P2RY1, purinergic receptor P2Y, G-protein coupled, 1, P2Y1, ATP recepto, P2 purinoceptor subtype Y1, P2Y purinoceptor 1, platelet ADP receptor, purinergic receptor P2Y1. Official Symbol: P2RY1. RefSeq number(s): NP_002554.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Arthur, Shen, Mu et al.: "Calmodulin interacts with the platelet ADP receptor P2Y1." in: The Biochemical journal, Vol. 398, Issue 3, pp. 339-43, 2006 (PubMed).