Pituitary Homeobox 3 (PITX3) antibody

Antigen: Pituitary Homeobox 3 (PITX3)

Synonyms: PTX3, CTPP4, MGC12766, ak, Ptx3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334461

Additional Information

Alternative name: PITX3

Gene ID: 5309

Immunogen: PEHGCKGQEHSD

Description: All names and symbols: PITX3, paired-like homeodomain 3, MGC12766, PTX3, homeobox protein PITX3. Official Symbol: PITX3. RefSeq number(s): NP_005020.1

Specificity: This antibody is not excpected to cross-react with PITX1 and PITX2. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9,282(45):33192-200., PMID: 17848564). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN334461 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kim, Inoue, Ishii et al.: "A MicroRNA feedback circuit in midbrain dopamine neurons." in: Science (New York, N.Y.), Vol. 317, Issue 5842, pp. 1220-4, 2007 (PubMed).