Patatin-Like phospholipase Domain Containing 2 (PNPLA2) antibody

Antigen: Patatin-Like phospholipase Domain Containing 2 (PNPLA2)

Synonyms: ATGL, TTS2, PEDF-R, FP17548, TTS-2.2, DESNUTRIN, DKFZp667M109, 1110001C14Rik, PNPLA2, pnpla2, LOC100219060

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334463

Additional Information

Alternative name: PNPLA2 / Desnutrin

Gene ID: 57104

Immunogen: C-HFNSKDELIQAN

Description: All names and symbols: PNPLA2, desnutrin, patatin-like phospholipase domain containing 2, 1110001C14Rik, ATGL, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase. Official Symbol: PNPLA2. RefSeq number(s): NP_065109.1

Specificity: Human, Mouse, Rat, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in Human Heart lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334463 (0.03µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Steinberg, Kemp, Watt: "Adipocyte triglyceride lipase expression in human obesity." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 4, pp. E958-64, 2007 (PubMed).