RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) antibody

Antigen: RAS Protein Activator Like 1 (GAP1 Like) (RASAL1)

Synonyms: RASAL, RASAL1, MRASAL, rasal1, MGC79606

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334473

Additional Information

Alternative name: RASAL

Gene ID: 8437

Immunogen: C-QERGKAALGPLGP

Description: All names and symbols: RASAL1, RASAL, RAS protein activator like 1 (GAP1 like), GAP1 like protein, RAS-GTPase-activating protein-like. Official Symbol: RASAL1. RefSeq number(s): NP_004649.1

Specificity: Human 804 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 90kDa band observed in Human Thyroid gland lysates (calculated MW of 90.0kDa according to NP_004649.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 21kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334473 (0.3µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Allen, Chu, Brill et al.: "Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog." in: Gene, Vol. 218, Issue 1-2, pp. 17-25, 1998 (PubMed).