Tumor Protein D52-Like 1 (TPD52L1) antibody

Antigen: Tumor Protein D52-Like 1 (TPD52L1)

Synonyms: D53, hD53, MGC8556, TPD52L2, TPD52L1, d53, MGC84190, MGC110003

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334515

Additional Information

Alternative name: TPD52L1 / D53

Gene ID: 7164

Immunogen: C-EPLQGTDEDAVASAD

Description: All names and symbols: TPD52L1, tumor protein D52-like 1, D53, MGC8556, TPD52L2, hD53, tumor protein D52-like 2, tumor protein D53. Official Symbol: TPD52L1. RefSeq number(s): NP_003278.1, NP_001003395.1 , NP_001003396.1, NP_001003397.1

Specificity: This antibody is expected to recognise isoforms 1, 3 and 4 (NP_003278.1, NP_001003396.1 and NP_001003397.1 resp.). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 26kDa band observed in Human Breast cancer lysates (calculated MW of 22.5kDa according to NP_003278.1). Recommended concentration: 0.1-0.3µg/ml. See also our D52 and D54 produ

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334515 (0.1 ug/ml) staining of Human Breast cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Boutros, Bailey, Wilson et al.: "Alternative splicing as a mechanism for regulating 14-3-3 binding: interactions between hD53 (TPD52L1) and 14-3-3 proteins." in: Journal of molecular biology, Vol. 332, Issue 3, pp. 675-87, 2003 (PubMed).