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DNA Topoisomerase II (TOP2) antibody
| Antigen | DNA Topoisomerase II (TOP2) |
| Synonyms | Topoii, top2 |
| Clonality | Polyclonal |
| Host |
Rabbit |
| Reactivity |
Arabidopsis thaliana |
| Application |
Western Blotting (WB), Immunolocalization (IL)
|
| Catalog no. | ABIN334538 |
| Quantity | 200 ul |
| Price | 473.20 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
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Additional Information
| Immunogen | The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II(At3g23890, protein accesion number P30182) with an N-terminal hexahistidinetag was expressed in E.coli and purified by Ni2+ affinity chromatography. |
| Format | Lyophilized |
| Description | Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzingunknotting of DNA by creating transient breaks in the DNA using a conservedtyrosine as the catalytic residue.Synonyme names of this protein: At3g23890,ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2,TOPOISOMERASE II |
| Specificity | Predicted reactivity: Arabidopsis thaliana. Not reactive in: Nicotiana tabacum. |
Application Details
| Application Notes | Recommended Dilution 1: 2000 (WB), 1: 500 (IL). Additional Information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thalianaprotein extracts. In subcellular fractions of cultured Arabidopsis cells the antibodydetects a 170 kDa protein exclusively in the nulear fraction (see picture). |
| Purity | Serum |
| Storage | store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes. |
| Research Area | Plant/Algal Physiology |
| Restrictions | For Research Use only |
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