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Antibody
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anti-DNA Topoisomerase II TOP2
DNA Topoisomerase II (TOP2) antibody
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN334538 |
| Quantity |
200 ul |
| Price |
473.20 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Immunogen
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The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II(At3g23890, protein accesion number P30182) with an N-terminal hexahistidinetag was expressed in E.coli and purified by Ni2+ affinity chromatography.
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Format
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Lyophilized
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Description
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Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzingunknotting of DNA by creating transient breaks in the DNA using a conservedtyrosine as the catalytic residue.Synonyme names of this protein: At3g23890,ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2,TOPOISOMERASE II
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Specificity
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Predicted reactivity: Arabidopsis thaliana. Not reactive in: Nicotiana tabacum.
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Application Notes
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Recommended Dilution 1: 2000 (WB), 1: 500 (IL). Additional Information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thalianaprotein extracts. In subcellular fractions of cultured Arabidopsis cells the antibodydetects a 170 kDa protein exclusively in the nulear fraction (see picture).
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Purity
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Serum
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Storage
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store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.
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Research Area
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Plant/Algal Physiology
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Restrictions
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For Research Use only
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Alternatives for antigen "DNA Topoisomerase II (TOP2)", type "Antibodies"
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