Vernalization Protein 2 (VRN2) antibody

Antigen: Vernalization Protein 2 (VRN2)

Clonality: Monoclonal

Host: Mouse

Reactivity: Wheat (Triticum)

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN334573

Additional Information

Immunogen: KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2sequence

Format: Lyophilized

Description: Plants with a winter growth habit flower earlier when exposed for several weeks tocold temperatures, a process called vernalization. The wheat vernalization geneVRN-2 is a dominant repressor of flowering that is down-regulated byvernalization. Loss of function of VRN-2, whether by natural mutations ordeletions, results in spring lines, which do not require vernalization to flower.

Specificity: Predicted reactivity: Triticum monococcum.

Application Details

Application Notes: Recommended Dilution 1: 1000 (WB), 1: 10 000 (ELISA). Additional Information: VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins inE. coli and purified through GST sepharose columns. The purified VRN-2 andZCCT2 proteins were used to test the specificity of the VRN-2 antibody byWestern blot analysis. A Western blot experiment showed that the VRN-2antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Purity: Affinity purified

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only