F-Box Protein 43 (FBXO43) antibody

Antigen: F-Box Protein 43 (FBXO43)

Synonyms: EMI2, ERP1, Fbx43, Emi2, MGC107440, 4930533G20Rik, emi2, erp1, Xerp1, MGC66488, fbxo43

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Catalog no.: ABIN335134

Additional Information

Alternative name: EMI2

Gene ID: 286151, 78803 (mouse), 315034 (rat)

Immunogen: Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001070996.1, NP_001025031.2

Description: All names and symbols: FBXO43, EMI2, F-box protein 43, Fbx43, early mitotic inhibitor 2, ERP1. Official Symbol: FBXO43. RefSeq number(s): NP_001070996.1, NP_001025031.2.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One. 2009 Oct 5,4(10):e7237. PMID: 19802389). Recommended concentration: 1-3µg/ml. This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One. 2009 Oct 5,4(10):e7237. PMID: 19802389). Recommended concentration: 5-10µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Perrard, Chassaing, Montillet et al.: "Cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes." in: PLoS ONE, Vol. 4, Issue 10, pp. e7237, 2009 (PubMed).