HRAS-Like Suppressor 3 (PLA2G16) antibody

Antigen: HRAS-Like Suppressor 3 (PLA2G16)

Synonyms: AdPLA, HRASLS3, HREV107, HREV107-3, MGC118754, H-REV107-1, Hrasls3, Hrev107, MLP-3, C78643, PLA2G16, MGC140776

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN335142

Additional Information

Alternative name: HRASLS3 / HREV107-3

Gene ID: 11145, 225845 (mouse), 24913 (rat)

Immunogen: RAPIPEPKPGDLIE-C

Description: All names and symbols: PLA2G16, phospholipase A2, group XVI, AdPLA, H-REV107-1, HRASLS3, HREV107, HREV107-3, MGC118754, HRAS-like suppressor 3, adipose-specific PLA2. Official Symbol: PLA2G16. RefSeq number(s): NP_009000.2

Specificity: Reported variants represent identical protein:NP_009000.2 and NP_001121675.1). Human, Mouse, Rat, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0kDa according to NP_009000.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only