PiggyBac Transposable Element Derived 1 (PGBD1) antibody

Antigen: PiggyBac Transposable Element Derived 1 (PGBD1)

Synonyms: SCAND4, HUCEP-4, dJ874C20.4, MGC102225, 4921509E05Rik, PGBD1, DKFZp469J1439

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN335157

Additional Information

Alternative name: PGBD1

Gene ID: 84547

Immunogen: C-EKWSHLSLTRRN

Description: All names and symbols: PGBD1 , piggyBac transposable element derived 1 , HUCEP-4, SCAND4, dJ874C20.4, OTTHUMP00000039508, cerebral protein-4. Official Symbol: PGBD1 . RefSeq number(s): NP_115896.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave bands at approx 150kDa and 17kDa in lysates of cell lines HeLa, HepG2 and K562 after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 92.5kDa according to NP_115896.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only