S100 Calcium Binding Protein A9 (S100A9) antibody

Antigen: S100 Calcium Binding Protein A9 (S100A9)

Synonyms: MIF, NIF, P14, CAGB, CFAG, CGLB, L1AG, LIAG, MRP14, 60B8AG, MAC387, Mrp14, S100A9, MGC137042

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN335161

Additional Information

Alternative name: S100A9

Gene ID: 6280

Immunogen: C-DTNADKQLSFEEF

Description: All names and symbols: S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B. Official Symbol: S100A9. RefSeq number(s): NP_002956.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 16kDa band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kDa) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280(50):41521-9., PMID: 16216873). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Innate Immunity

Restrictions: For Research Use only

Images

EB09146 (0.5µg/ml) staining of Human Peripheral Mononucleocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.