Cardiotin antibody

Catalog No. ABIN335361

Product Details

Target: Cardiotin

Reactivity: Human, Pig, Monkey, Cat, Rat, Dog, Xenopus laevis, Goat, Hamster, Mouse, Zebrafish (Danio rerio), Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human, monkey, swine, feline, canine, goat, hamster, mouse, rat, rabbit and Xenopus.

Purification: Purified

Immunogen: R2G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a mouse immunized with a total protein extract of chicken gizzard.

Clone: R2G

Isotype: IgM

Application Details

Application Notes: R2G reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. R2G is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays R2G reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution for immunoblotting applications should be determined by titration.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Pochampally, Neville, Schwarz, Li, Prockop: "Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9282-5, (2004) (PubMed).

Ausma, Litjens, Lenders, Duimel, Mast, Wouters, Ramaekers, Allessie, Borgers: "Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat." in: Journal of molecular and cellular cardiology, Vol. 33, Issue 12, pp. 2083-94, (2001) (PubMed).

Dispersyn, Geuens, Ver Donck, Ramaekers, Borgers: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, (2001) (PubMed).

Ausma, Wijffels, van Eys, Koide, Ramaekers, Allessie, Borgers: "Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation." in: The American journal of pathology, Vol. 151, Issue 4, pp. 985-97, (1997) (PubMed).

Schaart, Moens, Endert, Ramaekers: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).

Schaart, van der Ven, Ramaekers: "Characterization of cardiotin, a structural component in the myocard." in: European journal of cell biology, Vol. 62, Issue 1, pp. 34-48, (1994) (PubMed).

Target Details

Target: Cardiotin

Background: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.