Cardiotin antibody

Catalog No. ABIN335363

There is 1 publication for this product available. The Mouse Monoclonal anti-Cardiotin antibody is suitable to detect Cardiotin in samples from Human and Pig. It has been validated for WB, IHC (fro) and IHC.

Quick Overview for Cardiotin antibody (ABIN335363)

Target: Cardiotin

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Clone: SR-3

Product Details

Specificity: Human and swine.

Purification: Purified

Immunogen: SR-3 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the 100 kDa cardiotin subunit.

Isotype: IgM

Application Details

Application Notes: SR-3 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. SR-3 is useful for immunohistochemistry on frozen sections and immunoblotting. In immunoblotting assays SR-3 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Schaart, Moens, Endert, Ramaekers: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).

Target Details

Target: Cardiotin

Background: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.