Cardiotin antibody
The Mouse Monoclonal anti-Cardiotin antibody (Clone SR-4) (ABIN335364) specifically detects Cardiotin in WB, IHC (fro) and IHC.
The antibody is reactive with Human and Pig samples.
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Catalog No. ABIN335364
$548.46
Plus shipping costs $50.00
0.1 mg
Shipping to:
United States
Delivery in 4 to 7 Business Days
Quick Overview for Cardiotin antibody (ABIN335364)
Target
Cardiotin
Reactivity
Human, Pig
Host
Mouse
Clonality
Monoclonal
Conjugate
Un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
Clone
SR-4
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Specificity
- Human and swine.
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Purification
- Purified
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Immunogen
- SR-4 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cardiotin.
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Isotype
- IgM
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anti-Cardiotin antibody
Reactivity: Human, Pig, Cat, Dog, Goat, Hamster, Monkey, Mouse, Rat, Xenopus laevis, Zebrafish (Danio rerio), Rabbit WB, IHC (fro), IHC (p), IHC Host: Mouse Monoclonal R2G unconjugated
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Application Notes
- SR-4 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. SR-4 is useful for immunohistochemistry on frozen tissue sections and immunoblotting. In immunoblotting assays SR-4 reacts exclusively with the 300 kDa cardiotin protein complex. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Cardiotin
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Background
- Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Target
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