Nestin (NES) antibody

Antigen: Nestin (NES)

Synonyms: FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin

Clonality: Monoclonal (10C2)

Host: Mouse

Reactivity: Human

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335378

Additional Information

Alternative name: Nestin

Immunogen: Nestin (clone 10C2) is a monoclonal antibody derived by traditional hybridoma technology. A 150-amino-acid fragment from the cloned human nestin was conjugated to glutathione S-transferase and used as an immunogen.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 10C2

Description: Nestin is a Class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Specificity: 10C2 reacts exclusively with the human nestin protein. No cross-reactivity to rodent nestin.

Application Details

Storage: Can be stored at -20 o C to -70 o C for long term storage. Once thawed, store at 4 o C, do not refreeze.

Research Area: Angiogenesis, Lineage Markers

Restrictions: For Research Use only

Images

anti-Nestin (NES) antibody

Publications

Publications: Messam, Hou, Major: "Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody." in: Experimental neurology, Vol. 161, Issue 2, pp. 585-96, 2000 (PubMed).

Messam, Hou, Berman et al.: "Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells." in: Brain research. Developmental brain research, Vol. 134, Issue 01. Feb, pp. 87-92, 2002 (PubMed).

Mokry, Pudil, Ehrmann et al.: "Re-expression of nestin in the myocardium of postinfarcted patients." in: Virchows Archiv : an international journal of pathology, Vol. 453, Issue 1, pp. 33-41, 2008 (PubMed).