Laminin, alpha 1 (LAMA1) antibody

Antigen: Laminin, alpha 1 (LAMA1)

Synonyms: Lama, AA408497, LAMA, LAMA1

Clonality: Monoclonal (A5)

Host: Rat

Reactivity: Human, Mouse (Murine)

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN335387

Additional Information

Alternative name: Laminin

Immunogen: A5 is a rat monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a Fisher rat immunized with a laminin preparation from the EHS mouse tumor.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: A5

Description: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Laminin anchors cells to the basement membrane through interactions with integrins at the cell surface and collagens in the basement membrane.

Specificity: Human and mouse.

Application Details

Application Notes: A5 reacts with laminin. APPLICATIONS A5 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Neurology, Angiogenesis, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Ljubimov, Bartek, Couchman et al.: "Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies." in: International journal of cancer. Journal international du cancer, Vol. 50, Issue 4, pp. 562-6, 1992 (PubMed).