Lamin B2 (LMNB2) antibody

Antigen: Lamin B2 (LMNB2)

Synonyms: LMN2, LAMB2, MGC2721, lamin, RGD1563803, lmnb2-a, LMNB2, lmn2, lamb2, Lamin-L(II), LOC100136040

Clonality: Monoclonal (LN43)

Host: Mouse

Reactivity: Human, Mouse (Murine), Hamster, Xenopus laevis

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN335392

Additional Information

Alternative name: Lamin B2

Immunogen: LN43 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LN43

Description: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and lamin B2.

Specificity: Human, mouse, hamster and Xenopus.

Application Details

Application Notes: LN43 reacts with an epitope located in the C-terminal part of lamin B2. LN43 is suitable for immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Signaling, Cytoskeleton, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Hozuak, Sasseville, Raymond et al.: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995 (PubMed).

Jenkins, Hoelman, Lyon et al.: "Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix." in: Journal of cell science, Vol. 106 ( Pt 1), pp. 275-85, 1994 (PubMed).

Bridger, Kill, OFarrell et al.: "Internal lamin structures within G1 nuclei of human dermal fibroblasts." in: Journal of cell science, Vol. 104 ( Pt 2), pp. 297-306, 1993 (PubMed).

Machiels, Broers, Raymond et al.: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996 (PubMed).

Machiels, Zorenc, Endert et al.: "An alternative splicing product of the lamin A/C gene lacks exon 10." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9249-53, 1996 (PubMed).

Broers, Machiels, Kuijpers et al.: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).

Machiels, Ramaekers, Kuijpers et al.: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997 (PubMed).

Jansen, Machiels, Hopman et al.: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997 (PubMed).