Goat anti-Human IgG, Fc Antibody (Alkaline Phosphatase)

Antigen: IgG, Fc

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Alkaline Phosphatase (AP)

Application: Immunoassay (IA), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Catalog no.: ABIN336609

Additional Information

Alternative name: IgG Fc

Immunogen: Goat serum was obtained from animals of US origin and under the care of a registered veterinarian.

Description: This secondary antibody exihibits minimal cross reactivity with human IgA + IgM

Specificity: This antibody reacts with the heavy chains on human IgG based on IEP. Cross reactivity: No reactivity is observed with the light chains on human immunoglobulins based on IEP. No reactivity is observed to Human IgM or IgA based on IEP.

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry

Purity: Affinity purified using solid phase Human IgG

Purification: Affinity purified using solid phase Human IgG

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide

Storage: Store undiluted liquid at 2-8 °C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf Life:1 year from date of receipt

Restrictions: For Research Use only